Prashant P. Sharma
  • Home
  • Research
    • Phylogenomics >
      • Chelicerate phylogenomics
      • Scorpion phylogenomics
      • Sea spider phylogenomics
    • Evo-devo >
      • Harvestman Hox genes
      • Scorpion Hox genes
      • Evolution of the chelicera
      • RNAi in Phalangium opilio
      • Deutocerebral appendages
    • Genomics
    • Biodiversity discovery
    • Older (archived) projects >
      • Systematics >
        • Arthropod systematics >
          • Cyphophthalmi
          • Zalmoxidae
          • Basal Opiliones phylogeny
        • Laniatores
        • Bivalve systematics >
          • Basal bivalve phylogeny
          • Protobranch phylogeny
      • Biogeography >
        • Sandokanidae
        • Zalmoxoidea
        • Simulations and theory
  • Personnel
    • Join the lab
    • Jesús A. Ballesteros Chávez
    • Caitlin M. Baker
    • Guilherme Gainett
    • Andrew Z. Ontano
    • Emily V.W. Setton
  • Lab Photos
  • Publications
  • Fieldwork
    • Australia 2014
    • Philippines 2014
    • Laos 2014
    • Australia 2015
    • Colorado 2018
  • Protocols
  • Courses
Lab-specific Protocols
RNAi in Phalangium opilio (PDF)
This protocol covers techniques for dsRNA-mediated gene silencing in embryos of Phalangium opilio. Brief notes on embryo collection and cultivation are included.

Scorpion maintenance and dissection (PDF)
This protocol covers techniques and safety precautions for keeping scorpions in the laboratory, as well as dissection for embryo collection.

For more on developmental research with non-model arachnids, please see the Development and evo-devo tab.
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